Fig. 3

TBI alters expression of expression levels of surface molecules involved in DC functions. A–D Surface expression levels of CD80 (A), CD86 (B), MHC-CI (C) and MHC-CII (D) in total cDC, cDC1, cDC2 and pDC subsets of spleen from Sham (n = 4), d1- (n = 5) and d3- (n = 5) TBI mice. Shown were Geo-Mean Fluorescence Intensities (GMFI). E–G Surface expression levels of CD80 (E), CD86 (F) and MHC-CII (G) in total cDC, cDC1, cDC2 and pDC subsets of spleen from Sham (n = 4) and d7-TBI (n = 5) mice. Shown were GMFI. H, I Surface expression levels of CD80, CD86 and MHC-CII in PDCA1⁺CD11c⁺ pDC (H) and PDCA1⁻CD11c⁺ cDC (I) subsets of the BM from Sham (n = 4), d1- (n = 5) and d3- (n = 5) TBI mice. Shown were GMFI. All data represent mean ± SEM. Mann–Whitney non-parametric tests and two-tailed student’s t tests were used to assess statistical significance (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001)