Fig. 4

TBI results in an imbalance among DC subsets of the circulatory and lymphatic systems. A Relative frequencies of total cDC, cDC1 and cDC2 subsets in the peripheral blood from Sham (n = 4) and d7-TBI (n = 5) mice. Data are representative of three independent experiments. B Surface expression levels of MHC-CII in total cDC, cDC1 and cDC2 subsets of peripheral blood from Sham (n = 4) and d7-TBI (n = 5) mice. Shown were GMFI. Data are representative of three independent experiments. C Relative frequencies of total cDC, cDC1, cDC2 pDC subsets in the peripheral lymph nodes from Sham (n = 4) and d7-TBI (n = 5) mice. Data are representative of two independent experiments. D Surface expression levels of MHC-CII, CD80 and CD86 in total cDC, cDC1, cDC2 and pDC subsets of peripheral lymph nodes from Sham (n = 4) and d7-TBI (n = 5) mice. Shown were GMFI. Data are representative of two independent experiments. E Relative frequencies of total cDC, cDC1 and cDC2 subsets in the mesenteric lymph nodes from Sham (n = 4) and d7-TBI (n = 5) mice. Data are representative of two independent experiments. F Surface expression levels of MHC-CII, CD80 and CD86 in total cDC, cDC1, cDC2 and pDC subsets of mesenteric lymph nodes from Sham (n = 4) and d7-TBI (n = 5) mice. Shown were GMFI. Data are representative of two independent experiments. All data represent mean ± SEM. Two-tailed student’s t tests were used to assess statistical significance (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001)