Fig. 5

TBI affects DC distribution in solid organs. A Frequencies of CD11c⁺CII^high cDCs within pre-gated CD45⁺ hematopoietic fraction of lungs from Sham (n = 4) and d7-TBI (n = 5) mice. Data are representative of two independent experiments. B Frequencies of CD103⁺ cDC1 and CD11b⁺ cDC2 subsets within CD45⁺ CD11c⁺CII^high cDCs of lungs from Sham (n = 4) and d7-TBI (n = 5) mice. Data are representative of two independent experiments. C Frequencies of CD4⁺ cDC2 and CD4⁻ cDC2 subsets within CD45⁺ CD11c⁺CII^high CD103⁻CD11b⁺ cDC2 cells of lungs from Sham (n = 4) and d7-TBI (n = 5) mice. Data are representative of two independent experiments. D Frequencies of CD11c^intPDCA1⁺ pDCs within pre-gated CD45⁺ hematopoietic fraction of lungs from Sham (n = 4) and d7-TBI (n = 5) mice. Data are representative of two independent experiments. E Frequencies of CD11c⁺CII^high cDCs within pre-gated CD45⁺ hematopoietic fraction of liver from Sham (n = 4) and d7-TBI (n = 5) mice. Data are representative of two independent experiments. F Frequencies of CD103⁺ cDC1 and CD11b⁺ cDC2 subsets within CD45⁺ CD11c⁺CII^high cDCs of liver from Sham (n = 4) and d7-TBI (n = 5) mice. Data are representative of two independent experiments. G Frequencies of CD4⁺ cDC2 and CD4⁻ cDC2 subsets within CD45⁺ CD11c⁺CII^high CD103⁻CD11b⁺ cDC2 cells of liver from Sham (n = 4) and d7-TBI (n = 5) mice. Data are representative of two independent experiments. H Frequencies of CD11c^intPDCA1⁺ pDCs within pre-gated CD45⁺ hematopoietic fraction of liver from Sham (n = 4) and d7-TBI (n = 5) mice. Data are representative of two independent experiments. I Surface expression levels of MHC-CII in total cDC, cDC1, cDC2 and pDC subsets of liver from Sham (n = 4) and d7-TBI (n = 5) mice. Shown were GMFI. Data are representative of two independent experiments. J Frequencies of CD45⁺ hematopoietic cells from skin of Sham (n = 4) and d7-TBI (n = 5) mice. Data are representative of two independent experiments. K Frequencies of CD11c⁺CII^high cDCs within pre-gated CD45⁺ hematopoietic fraction of skin from Sham (n = 4) and d7-TBI (n = 5) mice. Data are representative of two independent experiments. L Frequencies of CD103⁺CD207⁺ dermal DCs within CD45⁺CD11c⁺CII^high cells of skin from Sham (n = 4) and d7-TBI (n = 5) mice. Data are representative of two independent experiments. M Frequencies of CD103⁻CD207⁺ Langerhans Cells within CD45⁺CD11c⁺CII^high cells of skin from Sham (n = 4) and d7-TBI (n = 5) mice. Data are representative of two independent experiments. All data represent mean ± SEM. Two-tailed student’s t tests were used to assess statistical significance (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001)