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Fig. 7 | Journal of Neuroinflammation

Fig. 7

From: Traumatic brain injury alters dendritic cell differentiation and distribution in lymphoid and non-lymphoid organs

Fig. 7

TBI results in diminished levels of ROS in DC subsets and Progenitors. A Histograms indicating intracellular ROS levels in CD11c+CII+ splenic cDCs from sham (n = 4), d1-TBI (n = 5) and d3-TBI (n = 5) mice. Shown were the frequencies of ROShigh cells under the indicated gates. Cells treated with DMSO served as negative controls. B Frequencies of gated ROShigh CD11c+CII+ splenic cDCs (top) and total intracellular ROS levels (GMFI) in CD11c+CII+ splenic cDCs (bottom) from sham (n = 4), d1-TBI (n = 5) and d3-TBI (n = 5) mice. C Histograms indicating intracellular ROS levels in CD8+CD11b−CD11c+CII+ splenic cDC1 subset from sham (n = 4), d1-TBI (n = 5) and d3-TBI (n = 5) mice. Shown were the frequencies of ROShigh cells under the indicated gates. Cells treated with DMSO served as negative controls. D Frequencies of gated ROShigh CD8+CD11b−CD11c+CII+ splenic cDC1 subset (top) and total intracellular ROS levels (GMFI) in CD8+CD11b−CD11c+CII+ splenic cDC1 subset (bottom) from sham (n = 4), d1-TBI (n = 5) and d3-TBI (n = 5) mice. E Histograms indicating intracellular ROS levels in CD8−CD11b+CD11c+CII+ splenic cDC2 subset from sham (n = 4), d1-TBI (n = 5) and d3-TBI (n = 5) mice. Shown were the frequencies of ROShigh cells under the indicated gates. Cells treated with DMSO served as negative controls. F Frequencies of gated ROShigh CD8−CD11b+CD11c+CII+ splenic cDC2 (top) and total intracellular ROS levels (GMFI) in CD8−CD11b+CD11c+CII+ splenic cDC2 (bottom) from sham (n = 4), d1-TBI (n = 5) and d3-TBI (n = 5) mice. G Histograms indicating intracellular ROS levels in CD8−CD11b−CD11c+CII+ splenic immature cDCs from sham (n = 4), d1-TBI (n = 5) and d3-TBI (n = 5) mice. Shown were the frequencies of ROShigh cells under the indicated gates. Cells treated with DMSO served as negative controls. H Frequencies of gated ROShigh CD8−CD11b−CD11c+CII+ splenic immature cDCs (top) and total intracellular ROS levels (GMFI) in CD8−CD11b−CD11c+CII+ splenic immature cDCs (bottom) from sham (n = 4), d1-TBI (n = 5) and d3-TBI (n = 5) mice. I Histograms indicating intracellular ROS levels in PDCA1+CD11c+ splenic pDCs from sham (n = 4), d1-TBI (n = 5) and d3-TBI (n = 5) mice. Shown were the frequencies of ROShigh cells under the indicated gates. Cells treated with DMSO served as negative controls. J Frequencies of gated ROShigh PDCA1+CD11c+ splenic pDCs (top) and total intracellular ROS levels (GMFI) in PDCA1+CD11c+ splenic pDCs (bottom) from sham (n = 4), d1-TBI (n = 5) and d3-TBI (n = 5) mice. K–O Histograms indicating intracellular ROS levels (GMFI) in total cDCs (K), cDC1 (L), cDC2 (M), immature cDCs (N) and pDCs (O) of the spleen from sham (n = 5) and d7-TBI (n = 5) mice. P Frequencies of gated ROShigh Lin−c-Kit+ Flt3+CD115+ CDPs (left) and total intracellular ROS levels (GMFI) in Lin−c-Kit+ Flt3+CD115+ CDPs (right) of the BM of Sham (n = 4), d1-TBI (n = 5) and d3-TBI (n = 5) mice. Data are representative of two independent experiments. All data represent mean ± SEM. Mann–Whitney non-parametric tests and two-tailed student’s t tests were used to assess statistical significance (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001)

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