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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: Neuroinflammatory transcriptional programs induced in rhesus pre-frontal cortex white matter during acute SHIV infection

Fig. 2

T cell resident immune signatures within the rhesus brain at homeostasis. A Regional expression of genes associated with T-cell populations in uninfected animals. Brackets indicate corresponding T-cell subsets associated with genes. B Sampling schematic indicating collection of cerebrospinal fluid (CSF), total brain parenchyma and deep cervical lymph node (dCLN) from a comparable group of uninfected rhesus macaques (ages 6 years [two animals], 11 years [one animal], and 16 years [one animal]; one male, three females) for flow cytometric analysis C–E. C t-SNE plots clustering of immune cell populations in the brain (left), CSF (middle), and dCLN (right) by phenotype. D Gating strategy for microglia (CD11b+CD45lo/int) (Left) and CD4/CD8 T cells (Right) in the brain, CSF, and dCLN. E Frequency of microglia and lymphocyte populations among total cells in rhesus macaque brain tissue. F Regional expression of cytokine genes in uninfected animals. Brackets indicate cytokine families. G Quantification of interleukin, CXCL-chemokines, and CCL-chemokines levels (expressed in Log2 Normalized Protein Expression [Log2NPX]) in the CSF of a cohort of uninfected animals (age 21–22 years; 8 females) [32]. A, F Data points indicate samples derived from brain region (indicated by color) and animal. *Adjusted p < 0.01 relative other synapse dense regions (STS, CN, and/or HP). Asterisk color corresponds to a region (CN [red]) for which a gene is differentially regulated relative to other synapse dense regions. Data points indicate individual animals E, G, with symbols indicating specific animals E. Bars in violin plots indicate quartiles and gray bars indicate limits of detection (E)

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