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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: T-cells in human trigeminal ganglia express canonical tissue-resident memory T-cell markers

Fig. 4

CD4 and CD8 T-cells recovered from human brain and trigeminal ganglia express a TRM phenotype. Mononuclear cells were isolated from paired peripheral blood (blood), normal appearing white matter (NAWM) and trigeminal ganglia (TG) of deceased brain donors (n = 8). Frequencies of CD69+, CD103+, CXCR6+ and CXCR3+ CD8 (a) and CD4 (b) T-cells were quantified by flow cytometry. Representative dot plots (left panels) and quantification (right panels) of CD69 and CD103 co-expression on CD8 (c) and CD4 (d) T-cells. Each dot represents data obtained from one individual; see Table 1 for reference symbols used for each brain donors, and bars show mean value. P values were calculated using Friedman test with Dunn’s multiple comparisons test between blood, NAWM and TG (not discriminating between HSV1− and HSV1+ TG). Mann–Whitney test was performed to compare TG-derived CD8 and CD4 T-cells recovered from HSV-1 naïve (HSV-1−) with latently HSV-1-infected individuals (HSV-1+). * p < 0.05; ** p < 0.001 and *** p < 0.0001. In c, d, all CD69/CD103 subsets from blood, NAWM, HSV1− and HSV1+ TG are compared with each other using two-way ANOVA with Tukey’s multiple comparisons test

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