Fig. 10

Inhibitors of neuroinflammation signaling reverse the changes in the glycolytic pathway induced by LPS in acute hippocampal slices. Glucose uptake was measured by a radioactivity assay. Lactate medium levels were evaluated by a spectrophotometric method. PFK1 activity was analyzed by kinetic assay. Downregulation of neuroinflammation reverses the glucose uptake effects. The effects of arundic acid (AA), an inhibitor of reactive astrocytes (A), minocycline (mino), an inhibitor of microglia cells (C), and MCC950 (MCC), an inhibitor of NLRP3 assembly (E), on glucose uptake were evaluated. The high lactate extracellular levels were reversed by AA (B) and mino (D). The specific inhibition of NLRP3 reverses the activity of PFK1 (G). Values are expressed as means ± standard error. Data were analyzed by ANOVA, followed by the Tukey test, assuming P < 0.05. * means significant increase when compared to sham group