Fig. 11

Inhibitors of neuroinflammation signaling reverse the effects of LPS on IL-1β and S100B in acute hippocampal slices. Pro-inflammatory immunocontents of IL-1β and the S100B protein were measured by ELISA. Astrocyte inhibition by arundic acid (AA) reversed the elevations in IL-1β and S100B (A-C). Microglia inhibition by minocycline (mino) only reversed the increase in IL-1β (D-F). NLRP3 inhibition by MCC950 (MCC) reversed the IL-1β and S100B secretion that was induced by LPS (H, I) and, when compared to LPS group, the intracellular immunocontent of IL-1β (G). Values are expressed as means ± standard error. Data were analyzed by ANOVA, followed by the Tukey test, assuming P < 0.05. * means significant increase, when compared to sham group