Fig. 8

Inhibitors of energetic metabolism reverse the effects of LPS on the glycolytic pathway in acute hippocampal slices. Glucose uptake was measured by a radioactivity assay. PFK1 activity was analyzed by kinetic assay. Lactate medium levels were evaluated by a spectrophotometric method. Metformin, a non-specific hexokinase inhibitor, increased glucose uptake per se, however it reversed the effects of LPS during co-incubation (A). Metformin per se increased extracellular lactate levels (C), without changing PFK1 activity (B) and did not affect such changes induced by LPS. The inhibitor of PFK1 (3PO), LDH (oxamic acid, OA), and aconitase (fluorocitrate, FLC) reversed the neurometabolic changes induced by LPS (D-L). Values are expressed as means ± standard error. Data were analyzed by ANOVA, followed by the Tukey test, assuming P < 0.05. *means significant increase when compared to sham group