Fig. 2

The focal DTH lesion. A Day 12 DTH lesion in the left striatum, stained for IBA1 (microglia/macrophages) with cresyl violet counter-staining. Scale bar = 200 μm. B On higher magnification, the lesion composed of a hypercellular core (within the inner black dashed line) and an outer active border (between the inner and outer black dashed lines). This outer active border consisted of a dense inflammatory infiltrate, which demonstrated a gradient of decreasing cellularity with increasing distance away from the lesion core. Perivascular cuffing and dilatation of vessels were also observed in this outer active border. Five non-overlapping representative fields (indicated by the red asterisks) at the medial edge of this outer active border were selected for IBA1 and GFAP-positive cell density quantification. Scale bar = 100 μm. DTH delayed-type hypersensitivity, GFAP glial fibrillary acidic protein, IBA1 ionized calcium binding adaptor molecule 1