Skip to main content
Fig. 6 | Journal of Neuroinflammation

Fig. 6

From: Metabolomics detects clinically silent neuroinflammatory lesions earlier than neurofilament-light chain in a focal multiple sclerosis animal model

Fig. 6

Top discriminatory serum and CSF metabolites. A VIP score ranking plot obtained from OPLS-DA modelling of serum CPMG spectral data at day 12, showing that allantoin and cytidine had the highest VIP scores. B DTH animals had lower serum concentrations of allantoin compared to control and naïve animals at day 12, indicating that low allantoin was specific for the presence of the DTH lesion. C Fold change in allantoin concentrations within DTH animals, normalized to the mean concentrations within controls at each time point (indicated by horizontal dashed line at 1.0), revealing that levels increased with time to that seen in controls. D Serum cytidine levels were lower in DTH animals at day 12 when compared to control and naïve animals although the difference between DTH and naïve animals did not reach statistical significance. E Normalized data showing that cytidine levels increased with time to that observed in control animals. F VIP score ranking plot derived from the CSF OPLS-DA models at day 12, showing that glutamine and glucose had the highest VIP scores. G Lower CSF glutamine concentrations were observed in DTH animals at day 12, compared to control and naïve animals. H Glutamine concentrations within DTH animals (normalized to controls) increased with time. I Glucose concentrations were also decreased in DTH animals although statistical significance was not reached on post hoc comparison between DTH and naïve animals. J Normalized glucose levels showing that glucose levels were increased at days 28 and 60, with a peak at day 28. ****p < 0.0001, ***p < 0.001, **p < 0.01, *p < 0.05 by post hoc Tukey test after one-way ANOVA. Data presented as mean ± SEM. ANOVA analysis of variance, AU arbitrary units, CPMG Carr–Purcell–Meiboom–Gill, DTH delayed-type hypersensitivity, OPLS-DA orthogonal partial-least square discriminant analysis, SEM standard error of mean, VIP variable importance in projection

Back to article page