Fig. 5

Mefenamic acid reverses the increase in TFAM and NRF1 gene expression and regulates Bax and Bcl-2 protein content in the brain of septic animals. Sepsis was induced by cecal ligation and perforation and mefenamic acid (10, 30 and 50 mg/kg) was administered immediately after sepsis induction. Twenty-four hours after rats were euthanized and the prefrontal cortex and hippocampus were isolated to the determination of the gene expression of A TFAM and B NRF-1. It was also measured protein content of C the pro-apoptotic Bax protein and D the anti-apoptotic Bcl-2 protein. Gene expression was quantified by the ∆Ct. Data are presented as mean ± SEM, with the statistical difference calculated using the one-way ANOVA test followed by Tukey's post hoc test. *p < 0.05 vs Sham + saline and ^#p < 0.05 vs CLP + saline. n = 5 per group