Fig. 5

PBMT-induced ROS generation in CD4⁺ T cells activated JAK2/STAT4/STAT5 signal pathway to upregulate the expression of IFN-γ/IL-10. A CD4 antibody was used to staining the CD4⁺ T cells, and then the flow cytometry was used to detect and quantitative analyze the generation of ROS (revealed by DCF) in WT, APP/PS1, and 3xTg-AD CD4⁺ T cells with or without PBMT (n = 4 per group). Cells were pre-incubated with N-acetyl cysteine (NAC, 1 mM) before PBMT. B, C Western blotting analysis (B) and quantification (C) of the expression of p-JAK2, p-STAT4, p-STAT5, T-bet, Foxp3 in T cells from WT, APP/PS1, and 3xTg-AD with or without PBMT (n = 3 per group). Some cells were pre-incubated with NAC or TG-101348 (JAK2 inhibitor, MCE, HY-10409, 1 μM) before PBMT. D, E The concentration of IFN-γ (D) and IL-10 (E) in T cells from WT, APP/PS1, and 3xTg-AD conditioned medium (CM) were detected by ELISA after PBMT (n = 4 per group). All quantifications are presented as mean ± SEM and were analyzed by One-way ANOVA test; ***p < 0.001, **p < 0.01, *p < 0.05 versus control group; ###p < 0.001, ##p < 0.01, #p < 0.05 versus indicated group