Skip to main content
Fig. 7 | Journal of Neuroinflammation

Fig. 7

From: TPM1 mediates inflammation downstream of TREM2 via the PKA/CREB signaling pathway

Fig. 7

TPM1 knockdown dysregulates the PKA/CREB pathway in the TREM2−/− retina. A, B DEGs related to the CREB signaling in neurons in WT (A) or TREM2−/− mice (B) following treatments with PBS, or with LPS and siTPM1-1 or siCTR. C–E Western bot analysis (C) and quantification of p-CREB and CREB (D, E) in WT or TREM2−/− mice following LPS or PBS treatment. Data are presented as mean ± SEM and analyzed by one-way ANOVA with Tukey’s multiple comparison test (compared to LPS + TREM2−/−, *p < 0.05, **p < 0.01; PBS + WT vs. LPS + WT, *p < 0.05). n = 4 mice in each group. F–K Western blot analysis (F) and quantification of p-PKA, PKA, p-CREB, CREB and TPM1 (G–K) in TREM2−/− mice after treatments with LPS and siTPM1-1 or siCTR. Data are presented as mean ± SEM and analyzed by one-way ANOVA with Tukey’s multiple comparison test (compared to PBS or LPS + siCTR, *p < 0.05, ***p < 0.001). n = 5 or 6 mice in each group. L–Q qPCR analysis of TPM1, TNF-α, IL-1β, IL-6, COX-2 and iNOS in TREM2−/− mice after treatments with LPS, siTPM1-1 and 666-15, a potent and selective CREB inhibitor. Data are presented as mean ± SEM and analyzed by one-way ANOVA with Tukey’s multiple comparison test (compared to LPS + siCTR or LPS + siTPM1-1, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001). n = 5 mice in each group

Back to article page