Fig. 3

I/R injury induced an expansion of RGC subclusters with ferroptosis. A t-SNE distribution showing 2 subclusters in RGC. B t-SNE distribution showing groups (Blank and I/R) in RGC. C Bar plots showing cell abundances across RGC-SCs (n = 2) for the Blank and I/R groups. D Violin plots showing the proportion of RGC-SCs in RGC between Blank and I/R groups (n = 3/group). P value were calculated using a Wilcoxon rank-sum test. E Heat map representing the scaled expression values of the top 10 genes defining each RGC-SCs. F GO and pathway enrichment analysis of the RGC-SCs clustering DEGs. P value was derived by a hypergeometric test. G Violin plots showing the expression of genes related to oxidative stress between Blank and I/R groups. H Violin plots showing the expression of genes related to regulated necrosis between Blank and I/R groups. I Violin plots showing the expression of genes related to ferroptosis between Blank and I/R groups