Fig. 4

I/R injury induced an expansion of Myeloid subclusters with ferroptosis. A GO and pathway enrichment analysis of the Myeloid cells clustering DEGs. P value was derived by a hypergeometric test. B Violin plots showing the inflammatory response score (up) and ferroptosis score (down) between Blank and I/R groups. C Heat map representing the scaled expression values of the top 10 genes defining each Myeloid subsets. D t-SNE distribution showing 4 subclusters in Myeloid. E t-SNE distribution showing groups (Blank and I/R) in Myeloid. F Bar plots showing cell abundances across Myeloid (n = 4) for the Blank and I/R groups. G Heat maps demonstrating up-regulated genes and pathways in Myeloid during retina I/R injury