Fig. 1

Mature neurons die by apoptosis in TD. TUNEL assay detect DNA fragmentation during apoptosis. A Fraction of TUNEL-labeled neurons after 4 days of B1 deprivation. Data were expressed as median ± interquartile range. Mann–Whitney test was used (*P ≤ 0.05). B Confocal microscopy images (×60) of OHCs show mature neurons (NeuN+) (in red), cell nuclei (DAPI) (in blue), and TUNEL (in green). In detail, a digital zoom (×5.5) of typical apoptotic neurons. CT controls, TD thiamine deficiency (scale bar = 50 μm)