Fig. 5

Relative expression of selected genes in OHCs cultured with or without B1. Fold change values were calculated from RT-qPCR data as 2^(−ΔΔCt), therefore, the dotted line indicates the basal mean expression level of the respective gene in the control group (CT). The means of the CT and thiamine deficiency (TD) groups were compared with the bidirectional analysis of variance (ANOVA) test followed by Tukey’s multiple comparisons test. CT and TD groups were also analyzed separately with Student’s t-test (P value in light gray) to identify possible statistical differences not detected by the multiple comparison method due to low statistical power of the small sample. Data were expressed as mean ± standard deviation (*P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001)