Fig. 7

RSV prevents microglia activation and promotes an earlier neurogenesis pulse in B1-deprived OHCs. A–C The means of activated microglia (A), microglia endpoints/branch length ratio (B), and neuronal density (C) of the CT (controls) and TD (thiamine deficiency) groups were compared with the bidirectional analysis of variance (ANOVA) test followed by the Tukey’s multiple comparisons test. Data were expressed as mean ± standard deviation (*P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001). The statistical differences represented by red horizontal bars and asterisks refer to the variation of neuronal density between TD groups treated with different doses of RSV. The gray horizontal bars and asterisks refer to the differences between CT and TD treated with the same doses of RSV. D Confocal microscopy images (×60) of OHC with markings of mature neurons (NeuN+) in red, cell nucleus (DAPI) in blue and activated microglia (Iba1+) in green (scale bar = 50 μm)