Skip to main content
Fig. 10 | Journal of Neuroinflammation

Fig. 10

From: GSK872 and necrostatin-1 protect retinal ganglion cells against necroptosis through inhibition of RIP1/RIP3/MLKL pathway in glutamate-induced retinal excitotoxic model of glaucoma

Fig. 10

GSK872 and Nec-1 attenuated glutamate-induced NLRP3 inflammasome activation. a Expression levels of NLRP3 and NLRP3 downstream proteins in R28 cells after exposure to 10 mM glutamate for 24 h with or without the addition of GSK872 or Nec-1. b Intravitreal administration of GSK872 or Nec-1 significantly decreased protein levels of NLRP3 and NLRP3 downstream proteins in NMDA-damaged eyes. c Representative immunofluorescence microphotographs of retinal sections stained with RBPMS (green), NLRP3 (red), and DAPI (blue) at 5 days post GSK872 and Nec-1 injection. Scale bar = 50 μm. GCL: ganglion cell layer; INL: inner nuclear layer; ONL: outer nuclear layer; CTL: the control group; Glu: glutamate. The results were recorded as mean ± SD from at least three independent experiments. **p < 0.01, ***p < 0.001, ****p < 0.0001 versus control or saline group; #p < 0.05, ##p < 0.01, ###p < 0.001 versus Glu + DMSO group or NMDA + DMSO group. ns: not significant

Back to article page

Journal of Neuroinflammation

ISSN: 1742-2094