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Fig. 6 | Journal of Neuroinflammation

Fig. 6

From: GSK872 and necrostatin-1 protect retinal ganglion cells against necroptosis through inhibition of RIP1/RIP3/MLKL pathway in glutamate-induced retinal excitotoxic model of glaucoma

Fig. 6

GSK872 and Nec-1 protected R28 cells from death triggered by glutamate. a CCK-8 assay of R28 cells treated with 10 mM glutamate and different concentrations of GSK872 for 24 h. b CCK-8 assay of R28 cells treated with 10 mM glutamate and different concentrations of Nec-1 for 24 h. c GSK872 and Nec-1 apparently blocked LDH release induced by glutamate in R28 cells. d Hoechst–PI dual staining assay and statistical analysis of PI-positive cells after 24 h treatment with 10 mM glutamate and 40 μM GSK872 or 20 μM Nec-1. Scale bar = 50 μm. CTL: the control group; Glu: glutamate. The results were recorded as mean ± SD from at least three independent experiments. ***p < 0.001, ****p < 0.0001 versus control; #p < 0.05, ##p < 0.01, ###p < 0.001, ####p < 0.0001 versus Glu + DMSO group. ns: not significant

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Journal of Neuroinflammation

ISSN: 1742-2094