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Fig. 7 | Journal of Neuroinflammation

Fig. 7

From: GSK872 and necrostatin-1 protect retinal ganglion cells against necroptosis through inhibition of RIP1/RIP3/MLKL pathway in glutamate-induced retinal excitotoxic model of glaucoma

Fig. 7

GSK872 and Nec-1 inhibited RGCs necroptosis and retinal injury in the glutamate-induced excitotoxic mouse model. a HE staining of retinal sections and quantitative measurement of total retinal thickness and GCC thickness after intravitreal administration of NMDA with GSK872 or Nec-1 for 5 days. Scale bar = 50 μm. b Immunofluorescence staining of retinal whole-mounts with RBPMS (green) and quantification of RBPMS-positive RGCs at 5 days post intravitreal injection of NMDA with GSK872 or Nec-1. RGCs were quantified in three areas (center, middle, periphery) in each of the four quadrants of the retina. Scale bar = 50 μm. GCL: ganglion cell layer; IPL: inner plexiform layer; INL: inner nuclear layer; OPL: outer plexiform layer; ONL: outer nuclear layer; GCC: ganglion cell complex. The results were recorded as mean ± SD from at least three independent experiments. ***p < 0.001, ****p < 0.0001 versus saline group; ##p < 0.01, ####p < 0.0001 versus NMDA + DMSO group. ns: not significant

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Journal of Neuroinflammation

ISSN: 1742-2094