Fig. 4

Trem2 ablation does not impair immune cell recruitment into the SC or myeloid cell activation. SC harvested from MHV–JHM-infected mice after PBS perfusion were used for RNA isolation and RT-PCR analysis. A–F Ccl2, Tnfa, Ifng, Il10, inos and Arginase1 mRNA expression monitored at different timepoint. Data are the mean ± SEM from 2 independent experiments (n = 4–7 mice/timepoint/experiment) and are analyzed by unpaired two-tailed Student t test and two-way ANOVA analysis followed by Bonferroni test. *p < 0.01, **p < 0.001, ***p < 0.0001. G, H Numbers of BMDM (CD45^hiCD11b⁺Ly6G⁻) and microglia (CD45^hiCD11b⁺Ly6G⁻) from SC of MHV–JHM-infected WT and Trem2^−/− mice were analyzed by flow cytometry at the indicated timepoints. I, J Surface expression of MHCII as assessed by mean fluorescent intensity (MFI) on BMDM or microglia. K–M Numbers of CD4, CD8 and virus-specific S510⁺ tetramer⁺ CD8 T cells from SCs of WT and Trem2^−/− mice. Data for flow cytometry are representative of three independent experiments, each with n = 3–4 individuals per timepoint and are analyzed by unpaired two-tailed Student t test and two-way ANOVA analysis followed by Bonferroni test