Fig. 7

Differentially expressed genes in microglia and BMDM from Trem2^−/− mice compared to WT mice. Microglia (CD45^intCD11b⁺) and BMDM (CD45^hiCD11b⁺Ly6G⁻) were purified from blood (day 0 pi), and SCs of infected WT and Trem2^−/− mice at days 12 and 28 pi by FACS; RNA was subjected to nCounter analysis using the myeloid cell probe panel. A, B Normalized count values of Trem2 and Tyrobp mRNA expression in microglia and BMDM from both mouse groups at indicated timepoints. Individual dots represent cells from an individual mouse for BMDM at day 12 pi and cells pooled from 2 to 3 mice at days 0 and 28 pi for both microglia and BMDM. Data show the mean ± SEM from 4 individual or 2–3 pooled samples per timepoint and are analyzed by unpaired two-tailed Student t test and two-way ANOVA analysis. C–H Volcano plots depict differentially expressed genes (p < 0.05) in microglia (C, E, G) and BMDM (D, F, H) from Trem2^−/− relative to WT mice at days 0, 12 and 28 pi. Trem2 was excluded from the volcano plots. Horizontal and vertical red lines guide p value < 0.05 and log2FC >|0.5, respectively