Fig. 10

Brainstem microglia exhibit phagocytotic activity at resting conditions. A Representative confocal photographs of DVC slices surface showing a typical distribution of fluorescent latex beads mostly lodged in the AP, the funiculus separans and the subpostremal NTS. Scale bars: 200 µm. B–C Maximal xyz projection (B) and 3D reconstruction (C) of confocal images of IBA1 immunostaining and latex bead fluorescence acquired in the funiculus separans area. Arrowheads indicate latex beads engulfed in IBA1+ processes. Scale bar: 10 µm. D. Serial Z-stacking confocal microscope images of latex bead phagocytosis (green dots) by IBA1 + microglia. Scale bar: 3 µm. AP area postrema; cc central canal; fs funiculus separans; NTS nucleus tractus solitarius