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Fig. 12 | Journal of Neuroinflammation

Fig. 12

From: Constitutively active microglial populations limit anorexia induced by the food contaminant deoxynivalenol

Fig. 12

Microglia inhibition by minocycline exacerbates DON-induced anorexia. A Representative images of CD68 staining in the DVC of animals treated with vehicle or minocycline. Scale bars: 200 µm. B Cumulative food intake (g) of control or minocycline-treated mice after receiving p.o. administration of vehicle (H2O) or DON 1.25 mg/kg BW for 24 h. C. Cumulative food intake (g), measured over a 6 h period, of control or minocycline-treated mice given having received p.o. or i.p. administration of DON 1.25 mg/kg BW. A 2-way repeated-measures ANOVA (p < 0.05) was performed followed by Bonferroni post hoc tests for individual time points. *Significantly different from respective control group, *p < 0.05, **p < 0.01, ***p < 0.001. #Significant difference between control and PLX3397 groups treated with DON; #p < 0.05; ###p < 0.001. D Microphotographs illustrating c-Fos protein labeling observed in DVC (left), PVN (middle) and ARC (right) of control or minocycline-treated mice that received either vehicle or DON 1.25 mg/kg BW and were killed 3 h after treatment. Scale bar: 200 µm. E Quantification of c-Fos positive cells in structures of interest for control and treated with DON mice. A 2-way repeated-measures ANOVA (P < 0.05) was performed followed by Bonferroni post hoc tests for individual time points. Differences in c-Fos expression between vehicle- and DON-treated groups were observed (*p < 0.05; ***p < 0.001). PLX3397 diet induced a significant difference between DON-treated groups (###p < 0.001). AP area postrema; ARC arcuate nucleus; cc central canal; DVC dorsal vagal dorsal; fs funiculus separans; ME median eminence; NTS nucleus tractus solitaries; PVN paraventricular nucleus; 3 V: third ventricle

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