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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Constitutively active microglial populations limit anorexia induced by the food contaminant deoxynivalenol

Fig. 3

PLX3397-induced microglia deletion exacerbates DON-induced anorexia. A Representative images of IBA1 staining in the dorsal vagal complex (DVC) of animal fed for 3 weeks with normal chow (NC) or food supplemented with PLX3397 (290 mg/kg chow). Scale bar: 100 µm. B–C Cumulative food intake (g), measured over a 24-h period, of control or PLX3397-treated mice given p.o. administration of either vehicle (H2O) or DON 12.5 (B) or 1.25 (C) mg/kg BW. A 2-way repeated-measures ANOVA (p < 0.05) was performed in B and C followed by post hoc Bonferroni tests for individual time points. Differences in food intake between vehicle- and DON-treated groups was observed (**p < 0.01, ***p < 0.001). PLX3397 diet induced a significant difference in food intake of DON-treated groups (##p < 0.01, ###p < 0.001). D. Microphotographs illustrating c-Fos protein labeling observed with DVC (left), PVN (middle) and ARC (right) of control or PLX3397 mice treated with either vehicle or DON 1.25 mg/kg BW and killed 3 h after treatment. Scale bar: 200 µm. E. Quantification of c-Fos-positive cells in structures of interest. A 2-way repeated-measures ANOVA (p < 0.05) was performed followed by post hoc Bonferroni tests for individual time points. Differences in c-Fos expression between vehicle- and DON-treated groups was observed (***p < 0.001). The PLX3397 diet induced a significant difference between DON-treated groups (##p < 0.01, ###p < 0.001). AP area postrema; ARC arcuate nucleus; cc central canal; DVC dorsal vagal complex; ME median eminence; NTS nucleus tractus solitarius; PVN paraventricular nucleus; 3 V: third ventricle

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