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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: Constitutively active microglial populations limit anorexia induced by the food contaminant deoxynivalenol

Fig. 5

CD68+ microglia are present within the DVC and hypothalamus of control animals. A Representative photomicrographs of CD68 labeling performed on brainstem coronal sections of control mice. Note the localized expression of CD68 within the funiculus separans and postremal NTS. Lower panels High magnification images of double IBA1/CD68 labeling highlighting the presence of different microglial cell phenotypes within the AP, funiculus separans and NTS. The white box indicated regions where inset originated. B Representative photomicrographs of CD68 labeling performed on hypothalamic coronal sections of control mice. Note the localized expression of CD68 within the ARC/ME border. Lower panel High magnification images of IBA1/CD68 double-labeling highlighting the presence of different microglial cell phenotypes within the ARC and ME. The white box at A indicated the regions where inset originated. C High magnification images of double CD68/TMEM119 labeling performed at the funiculus separans level. Scale bars: 200 µm in A–B, 50 µm in C. ARC arcuate nucleus; AP area postrema; cc central canal; fs funiculus separans; ME median eminence; NTS nucleus tractus solitaries; 3 V, third ventricle; XII: hypoglossal nucleus

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