Table 1 Immunohistochemistry conditions
Immunostaining | Primary antibody | Secondary antibody | Serum blocking |
|---|---|---|---|
IBA-1 | Goat polyclonal anti-IBA1 (1:500) ab5076, Abcam | Donkey anti-Goat Alexa Fluor 488 (1:400) A11055, Invitrogen | Horse serum 3% |
c-Fos | Rabbit polyclonal anti-c-Fos (1:3000) ABE457, Merck Millipore | Biotinylated Goat anti-Rabbit (1:400) BA-1000, Vector Labs | Normal goat serum 3% |
IBA-1/CD68 | Rabbit polyclonal anti-IBA1 (1:4000) 019-19741, Fujifilm Wako Mouse monoclonal anti-CD68 (1:200) E-AB-22013, Elabscience | Donkey anti-Rabbit Alexa Fluor 594 (1:400) A21207, Invitrogen Goat anti-Mouse Alexa Fluor 488 (1:400) A11029, Invitrogen | Bovine serum albumine 3% Bovine serum albumine 3% |
IBA-1/CD206 | Rabbit polyclonal anti-IBA1 (1:4000) 019-19741, Fujifilm Wako Goat polyclonal anti-CD206 (1:500) AF2535, R&D Systems | Donkey anti-Rabbit Alexa Fluor 594 (1:400) A21207, Invitrogen Donkey anti-Goat Alexa Fluor 488 (1:400) (A11055, Invitrogen) | Bovine serum albumine 3% Bovine serum albumine 3% |
IBA-1/CD11b | Rabbit polyclonal anti-IBA1 (1:4000) 019-19741, Fujifilm Wako Rat monoclonal anti-CD11b (1:100) Sc-23937, Santa Cruz Biotechnology | Donkey anti-Rabbit Alexa Fluor 488 (1:400) A21206, Invitrogen Goat anti-Rat Alexa Fluor 594 (1:400) A11007, Invitrogen | Bovine serum albumine 3% Bovine serum albumine 3% |
CD11b/CD68 | Rat monoclonal anti-CD11b (1:100) Sc-23937, Santa Cruz Biotechnology Mouse monoclonal anti-CD68 (1:200) E-AB-22013, Elabscience | Goat anti-Rat Alexa Fluor 594 (1:400) A11007, Invitrogen Goat anti-Mouse Alexa Fluor 488 (1:400) A11029, Invitrogen | Bovine serum albumine 3% Bovine serum albumine 3% |
IBA-1/TMEM119 | Goat polyclonal anti-IBA1 (1:500) ab5076, Abcam Rabbit polyclonal anti-TMEM119 (1:2000) GTX134087, GeneTex | Donkey anti-Goat Alexa Fluor 488 (1:400) A11055, Invitrogen Donkey anti-Rabbit Alexa Fluor 594 (1:400) A21207, Invitrogen | Horse serum 3% Bovine serum albumine 3% |
TMEM119/CD68 | Rabbit polyclonal anti-TMEM119 (1:2000) GTX134087, GeneTex Mouse monoclonal anti-CD68 (1:200) E-AB-22013, Elabscience | Donkey anti-Rabbit Alexa Fluor 594 (1:400) A21207, Invitrogen Goat anti-Mouse Alexa Fluor 488 (1:400) A11029, Invitrogen | Bovine serum albumine 3% Bovine serum albumine 3% |
FG/CD68 | Rabbit polyclonal anti-FG (1:1000) AB153, Chemicon Mouse monoclonal anti-CD68 (1:200) E-AB-22013, Elabscience | Donkey anti-Rabbit Alexa Fluor 594 (1:400) A21207, Invitrogen Goat anti-Mouse Alexa Fluor 488 (1:400) A11029, Invitrogen | Normal Goat serum 3% Bovine serum albumine 3% |
FG/IBA-1 | Rabbit polyclonal anti-FG (1:1000) AB153, Chemicon Goat polyclonal anti-IBA1 (1:500) ab5076, Abcam | Donkey anti-Rabbit Alexa Fluor 594 (1:400) A21207, Invitrogen Donkey anti-Goat Alexa Fluor 488 (1:400) (A11055, Invitrogen) | Normal Goat serum 3% Horse serum 3% |