Fig. 7

Protection against BPRA injury by switching microglia from a pro-inflammatory to anti-inflammatory phenotype in AR-/- mice. A, B Representative images of CD16/32 and Arginase 1 IF staining with quantification of Iba1-positive microglia. C, D Summary of number changes in ipsilateral spinal CD16/32⁺ and Arginase1⁺ cells colocalization-positive to microglia between AR^−/− and WT mice. E Summary of changes in ipsilateral spinal IL-1β, IL-6, ICAM1, and IL-10 levels between AR^−/− and WT mice. F Summary of changes in ipsilateral spinal IL-1β, IL-6, Arginase 1, and IL-10 mRNA levels between AR^−/− and WT mice. Data were analyzed by two-way ANOVA and are presented as the mean ± SEM, n = 5 mice/group, ^####p < 0.0001, WT vs. sham group, ^{^^^^}p < 0.0001, AR^−/− vs. sham group, ****p < 0.0001, WT vs. sham group. Scale bar = 50 μm (A, B)