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Fig. 9 | Journal of Neuroinflammation

Fig. 9

From: Motor neuron survival is associated with reduced neuroinflammation and increased autophagy after brachial plexus avulsion injury in aldose reductase-deficient mice

Fig. 9

Testing the efficacy of pharmacological inhibition of AR in attenuating BPRA-induced MNs death in mice. A (a–d) Representative IF staining images of AR, ChAT, GAP43, and C-Caspase3. (e–h) Number changes in ipsilateral ChAT+ MNs and AR+/NeuN+, GAP43+/NeuN+ and C-Caspase3+/NeuN+ colocalization-positive neurons between the BPRA and BPRA + EPAL groups in WT mice. B (a–d) Representative images of SIRT1, p-AMPK, LC3B and P62 IF staining with NeuN. (e–h) Number changes in SIRT1+/NeuN+, p-AMPK+/NeuN+, LC3B+/NeuN+ and P62+/NeuN+ colocalization-positive neurons between the BPRA and BPRA + EPAL groups in WT mice. C (a–d) Representative images of Iba1 and GFAP IF staining with NeuN and CD16/32 and Arg1 IF staining with Iba1. (e–h) Changes in Iba1 and GFAP average fluorescence intensity and number changes CD16/32+ and Arg1+ cells colocalization to Iba-1+ cells between the BPRA and BPRA + EPAL groups in WT mice. All data were analyzed by two-way ANOVA and are presented as the mean ± SEM, n = 5 mice/group, ####p < 0.0001, BPRA vs. sham group, ^^^^p < 0.0001, BPRA + EPAL vs. sham + EPAL group, ****p < 0.0001, BPRA + EPAL vs. BPRA group. Scale bar = 50 μm

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