Fig. 1

Buffering nuclear calcium in neurons enhances the accumulation of microglia 3 h after TBI. A, B Significant increase of neuronal pCREB intensity 3 h post-TBI compared to Sham (CS vs CT; 1.00 ± 0.01 vs 2.11 ± 0.44). Buffering of nuclear Calcium significantly decreases neuronal pCREB intensity 3 h post-TBI (CT vs PT; 2.11 ± 0.44 vs 1.37 ± 0.35). Red datapoints indicate average per animal (N = 4, used for statistics); smaller datapoints depict individual neurons. Mean ± SD. C Neuronal density at the injury site 3 h post-TBI; no difference across groups. N = 4. D, E Unchanged microglial pCREB intensity 3 h post-TBI compared to sham. Buffering of nuclear calcium does not significantly increase microglial pCREB intensity 3 h post-TBI. N = 4. F Buffering of nuclear Calcium significantly increased microglia density upon TBI (CT vs PT; 7.65 ± 3.65 vs 15.66 ± 3.81). No significant difference in microglia density in animals injected with control virus (CS vs CT). Data are shown as mean ± SD. N = 4 mice. Scale bar: 25 µm. *p < 0.05; ***p < 0.001