Fig. 3From: Genetic deletion of Krüppel-like factor 11 aggravates traumatic brain injuryGenetic deletion of KLF11 aggravates brain tissue loss in mice after TBI. KLF11 KO and WT mice were subjected to TBI or sham operation, followed by a 30-d survival period. Tissue loss was examined in brain sections at 30 d after operation by using MAP2 immunostaining. A Representative images of MAP2 immunostaining (Bregma from + 0.98 mm to − 1.58 mm). B, C Quantitative analysis of total volume of brain atrophy and tissue atrophy area in each brain slice (n = 11–12/group, unpaired t-test). Neuronal loss was examined by Cresyl Violet (CV) staining and NeuN immunostaining in brain sections at 30 d after TBI. D Representative images of CV staining in the peri-lesional cerebral cortex (CTX), hippocampal CA1, and hippocampal CA3 regions. E Peri-lesional brain areas (rectangles) in the CTX, CA1, and CA3 where images in D and I were captured. F–H Quantitative analysis of CV-stained neurons in the peri-lesional CTX, CA1, and CA3 regions (n = 6/group, one-way ANOVA with Tukey post hoc test). I Representative images of NeuN immunostaining in the peri-lesional CTX, CA1, and CA3 regions. J–L Quantitative analysis of NeuN-immunopositive neurons in the peri-lesional CTX, CA1, and CA3 regions (n = 6/group, one-way ANOVA with Tukey post hoc test). M Correlation analysis between sensorimotor or cognitive outcome and CV-stained/NeuN-positive neurons in the peri-lesional CTX, CA1, and CA3 regions (n = 6/group, Pearson correlation analysis). Data are presented as mean ± SD. *p < 0.05, **p < 0.01 or ***p < 0.001 versus TBI + WT groupBack to article page