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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Genetic deletion of Krüppel-like factor 11 aggravates traumatic brain injury

Fig. 3

Genetic deletion of KLF11 aggravates brain tissue loss in mice after TBI. KLF11 KO and WT mice were subjected to TBI or sham operation, followed by a 30-d survival period. Tissue loss was examined in brain sections at 30 d after operation by using MAP2 immunostaining. A Representative images of MAP2 immunostaining (Bregma from + 0.98 mm to − 1.58 mm). B, C Quantitative analysis of total volume of brain atrophy and tissue atrophy area in each brain slice (n = 11–12/group, unpaired t-test). Neuronal loss was examined by Cresyl Violet (CV) staining and NeuN immunostaining in brain sections at 30 d after TBI. D Representative images of CV staining in the peri-lesional cerebral cortex (CTX), hippocampal CA1, and hippocampal CA3 regions. E Peri-lesional brain areas (rectangles) in the CTX, CA1, and CA3 where images in D and I were captured. FH Quantitative analysis of CV-stained neurons in the peri-lesional CTX, CA1, and CA3 regions (n = 6/group, one-way ANOVA with Tukey post hoc test). I Representative images of NeuN immunostaining in the peri-lesional CTX, CA1, and CA3 regions. JL Quantitative analysis of NeuN-immunopositive neurons in the peri-lesional CTX, CA1, and CA3 regions (n = 6/group, one-way ANOVA with Tukey post hoc test). M Correlation analysis between sensorimotor or cognitive outcome and CV-stained/NeuN-positive neurons in the peri-lesional CTX, CA1, and CA3 regions (n = 6/group, Pearson correlation analysis). Data are presented as mean ± SD. *p < 0.05, **p < 0.01 or ***p < 0.001 versus TBI + WT group

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