Fig. 4

KLF11 genetic deficiency exacerbates white matter injury in mice after TBI. Luxol fast blue (LFB) histological staining and MBP/SMI32 double-immunostaining were applied to detect white matter integrity in KLF11 KO and WT mouse brains at 30 d after TBI. A Representative LFB images of the pericontusional CTX, external capsule (EC), and striatum (STR) regions. B Pericontusional brain areas (rectangles) in the CTX, EC, and STR where images in A, D, and H were captured. C Quantitative analysis of relative OD values of LFB in pericontusional CTX, EC, and STR regions (n = 6/group, one-way ANOVA with Tukey post hoc test). D Representative images of MBP (green) and SMI32 (red) double-immunostaining in pericontusional CTX, EC, and STR regions. Quantitative analysis of MBP fluorescence intensities (E), SMI32 fluorescence intensities (F), and the ratio of SMI32/MBP (G) in the pericontusional CTX, EC, and STR regions (n = 6/group, one-way ANOVA with Tukey post hoc test). The nodes of Ranvier (NOR) were double-immunostained by Caspr/Nav1.6. H Representative images of Caspr (Red)/Nav1.6 (green) in the pericontusional EC region (rectangles: enlarged area of low-magnification images). I An image showing the composition and normal structure of the nodes of Ranvier. Quantitative analysis of the number of NORs (J), paranode length (K), and length of paranode gaps (L). M Correlation analysis of sensorimotor or cognitive outcome and white matter integrity (n = 6/group, Pearson correlation analysis). Data are presented as mean ± SD. *p < 0.05, **p < 0.01 or ***p < 0.001 versus TBI + WT group