Fig. 5

KLF11 genetic deficiency promotes astrocytic activation in the white and grey matter of mice after TBI. KLF11 KO and WT mice were subjected to TBI or sham operation and reactive astrocytes were detected in brain sections at 3 d after surgery by GFAP immunostaining. A–C Representative images of GFAP (green) and DAPI (blue) in the peri-lesional CTX (A), EC (B), and STR (C) regions (rectangles: enlarged area of low-magnification images). D–F Quantitative analysis of GFAP-positive astrocytes in the peri-lesional CTX (D), EC (E), and STR (F) regions. Data are presented as mean ± SD, n = 6/group. Statistical analyses were performed by one-way ANOVA with the Tukey post hoc test. *p < 0.05, **p < 0.01 or ***p < 0.001 versus TBI + WT group