Fig. 6

MyD88-TLR4 dependence of the CP response after striatal whole-blood injection. A KEGG-2019 mouse-based gene-set enrichment patterns in the CP and the striatum (STR) (control as baseline, n = 6). B Temporal dynamics of the MyD88-dependent-TLR4-signaling gene set score in the CP (n = 6). C Spatial projection of the MyD88-dependent-TLR4-signaling gene set score 24 h after injection. D ICAM1 signal intensity 24 h after striatal NaCl (sham, n = 6) or whole-blood (n = 6) injection in littermate TLR4 + / + mice (wt), and whole-blood injection in TLR4−/− (TLR4ko) mice (n = 6). Top: ICAM1 density-maps with a CP zoom-in. Bottom: Mean ICAM1 signal intensity in different anatomical compartments. LV, lateral ventricle; 3rdV, third ventricle. E ICAM1 signal intensity 24 h after striatal NaCl (sham) or whole-blood injection in littermate MyD88 + / + mice (wild-type), and whole-blood injection in MyD88 knockout (MyD88ko) mice (n = 8 per group). Top: ICAM1 density-maps with a CP zoom-in. Bottom: Mean ICAM1 signal intensity in different anatomical compartments. F F4/80 signal intensity 24 h after striatal NaCl (sham) or whole-blood injection in littermate TLR4 + / + mice (wild-type), and whole-blood injection in TLR4 knockout (TLR4ko) mice (n = 6 per group). Top: F4/80 density-maps with a CP zoom-in. Bottom: Mean F4/80 signal intensity in different anatomical compartments. G F4/80 signal intensity 24 h after striatal NaCl (sham or whole-blood injection in littermate MyD88 + / + mice (wt), and whole-blood injection in MyD88−/− (MyD88ko) mice (n = 8 per group). Top: F4/80 density-maps with a CP zoom-in. Bottom: Mean F4/80 signal intensity in different anatomical compartments