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Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: Recombinant human plasma gelsolin reverses increased permeability of the blood–brain barrier induced by the spike protein of the SARS-CoV-2 virus

Fig. 1

Plasma gelsolin (pGSN) significantly reduces disruption of the blood–brain barrier caused by the SARS-CoV-2 Spike protein S1 subunit in the 2D Transwell permeability assay. Confluent monolayer of human cerebral microvascular endothelial cells (hCMEC/D3) seeded on a Transwell semi-permeable membrane exposed to tested compounds added to the upper chamber (blood) (Panel Ai). The functional state of the cells as a barrier was evaluated with transendothelial electrical resistance (TEER) measurement and Dextran-FITC permeability assay (Panel Aii). The dextran-FITC intensity was measured in the lower chamber (brain) that migrated from the upper chamber (blood) in time (Panels B, D). Change in TEER was measured using Epithelial Voltohmeter EVOM2 (Panels C and D). The data represent the mean ± SEM of four (n = 4, with 2 inserts used per condition each time) independent experiments. * and ^ indicate statistical significance at p ≤ 0.05 compared to CT and S1, respectively, by one-way ANOVA and Tukey post hoc test

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