Fig. 2

circ_0001293 sponges miR-8114, and miR-8114 targets TGF-β2. a Schematic representation of the miR-8114 binding site in circ_0001293-WT and circ_0001293-MUT. b Schematic representation of the miR-8114 binding site in TGF-β2-WT and TGF-β2-MUT. c Dual-luciferase reporter assays were performed and showed that miR-8114 targets circ_0001293. ^***P < 0.001 compared with miR NC. d RT–qPCR was conducted to determine the expression of miR-8114. ^***P < 0.001 compared with NC. e Dual-luciferase reporter assays were conducted and showed that miR-8114 targets TGF-β2. ^***P < 0.001 compared with miR NC. f RT–qPCR was used to determine TGF-β2 expression. ^***P < 0.001 compared with NC. g RT–qPCR was performed to determine the expression of circ_0001293, miR-8114 and TGF-β2. ^*P < 0.05 and ^***P < 0.001 compared with NC, ^###P < 0.001 compared with oe-circ_0001293, and ^&&&P < 0.001 compared with si-circ_0001293. h Levels of the TGF-β2 protein detected using Western blotting. ^**P < 0.01 compared with NC; ^#P < 0.05 compared with oe-circ_0001293. All values are presented as the means ± SD (n = 3)