Fig. 8
HsTx2 alleviates the progression of epilepsy via the circ_0001293/miR-8114/TGF-β2 axis. a RT–qPCR was used to determine the expression of circ_0001293, miR-8114 and TGF-β2. b Levels of the TGF-β2 protein were examined using Western blotting. c Representative EEG recordings from the five groups of mice and histogram of the number of spike waves. d Levels of the TGF-β2 protein were examined using immunofluorescence staining. e ELISAs were used to determine the levels of TNF-α, IFN-γ, IL-6, IL-10 and TGF-β2. f Levels of MAPK- and NF-κB-related proteins were determined using Western blotting. ***P < 0.001 compared with the NC group; #P < 0.05 and ###P < 0.001 compared with the PTZ group; &P < 0.05, &&P < 0.01 and &&&P < 0.001 compared with the PTZ + HsTx2 group. All values are presented as the means ± SD (n = 6)