Fig. 1

APOE4 and female sex reduce protective astrocyte interactions with amyloid plaques. A Representative confocal images of THK-265-positive amyloid plaques (cyan), GFAP-immunoreactive astrocytes (green), LAMP1-positive dystrophic neurites (magenta) and cell nuclei labeled with 4′,6-diamidino-2-phenylindole (dark blue) in male and female E3FAD and E4FAD mice. Scale bars = 10 μm. Amyloid plaques were quantified for B coverage with GFAP-immunoreactive astrocyte processes, C plaque circularity, D plaque area, and E LAMP1-positive dystrophic neurites (normalized to plaque area) in male (E3M) and female (E3F) E3FAD and male (E4M) and female (E4F) EFAD mice. Data are presented as box (mean and 25th and 75th quartiles) and whisker (minimum and maximum values) plots from n = 6/group of mice at age 6 months. *p < 0.05, **p < 0.01, ***p < 0.001