Fig. 1

Ramified macrophages re-tile the retina after widespread photoreceptor degeneration. A Representative in vivo retinal imaging to track retinal thinning (OCT) and macrophages (SLO) in an Arr1^−/−Cx3cr1^+/GFP mouse. B Maximum intensity projections through ~ 10 μm the inner plexiform layer of Arr1^+/+Cx3cr1^+/GFP (WT) and Arr1^−/−Cx3cr1^+/GFP (KO) retinas after 20 days of light exposure. Retinal flatmounts were labeled for GFP (green) and MHCII (orange) using IHC. C Maximum intensity projections through the inner plexiform layer of C57BL/6J (WT) and Arr1^−/− (KO) after 20 days of light exposure, pulsed with EdU at 2 and 3 days of light exposure. Macrophages are labeled with CD11b (red) and EdU⁺ nuclei are indicated in green. D Quantification of C, showing the percentage of EdU⁺ CD11b⁺ cells out of all CD11b⁺ cells in the inner plexiform layers of KO and WT retinas, pulsed with EdU at 2 and 3 days, probed at 20 days of light exposure. Percentages displayed as mean ± SE; n = at least 7 images per genotype; ***p < 0.001. ONL = outer nuclear layer; INL = inner nuclear layer; IPL = inner plexiform layer. Scale bars indicate 50 μm