Table 1 Antibodies and labels used for immunohistochemistry and flow cytometry
Reagent | Manufacturer | Catalog number | RRID | Application | Dilution |
|---|---|---|---|---|---|
Goat anti-GFP antibody | Rockland | 600-101-215 | AB_218182 | IHC | 1:200 |
Alexa Fluor 647 anti-CD11b antibody | Biolegend | 101218 | AB_389327 | IHC | 1:300 |
Alexa Fluor 594 anti-I-A/I-E (MHCII) antibody | Biolegend | 107650 | AB_2566438 | IHC | 1:200 |
Alexa Fluor 647 anti-I-A/I-E (MHCII) antibody | Biolegend | 107617 | AB_493526 | IHC | 1:200 |
Rabbit anti-RFP antibody | Rockland | 600-401-379 | AB_2209751 | IHC | 1:750 |
Click-iT EdU kit for imaging, Alexa Fluor 488 | Invitrogen | C10337 | None | IHC | NA |
Zombie NIR Viability Dye | Biolegend | 423105 | None | FC | 1:500 |
APC anti-CD45 antibody | Biolegend | 103112 | AB_312977 | FC | 1:100 |
PE anti-CD45 antibody | Biolegend | 103106 | AB_312971 | FC | 1:100 |
BV605 anti-CD11b antibody | Biolegend | 101257 | AB_2565431 | FC | 1:100 |
BV711 anti-I-A/I-E antibody | Biolegend | 107643 | AB_2565976 | FC | 1:100 |
Alexa Fluor 488 anti-Ly6C antibody | Biolegend | 128022 | AB_10639728 | FC | 1:100 |
BV421 anti-CX3CR1 antibody | Biolegend | 149023 | AB_2565706 | FC | 1:100 |