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Fig. 6 | Journal of Neuroinflammation

Fig. 6

From: Microglial diversity along the hippocampal longitudinal axis impacts synaptic plasticity in adult male mice under homeostatic conditions

Fig. 6

Variations in microglial morphology and ultrastructure in the DH versus VH CA1 stratum radiatum. Representative brightfield images at a ×40 magnification showing IBA1+ microglia in the dorsal (A) and ventral (B) CA1 stratum radiatum (SR). The soma (C) and manual arbor perimeters (D), as well as the automatic arbor solidity (E), manual arbor circularity (F) and lacunarity (G), were calculated for IBA1+ cells in the DH and VH using brightfield microscopy (n = 6 mice). The mean relative number of microglial cell bodies containing immature (H) and mature (I) lysosomes per animal were determined using scanning electron microscopy (SEM) (n = 4 mice). Representative SEM of microglia in the dorsal (J, K) and ventral (L, M) CA1 SR. SEM images are pseudocolored as follows: light blue, microglia; grey, microglial nucleus; green, extracellular digestions; beige, dendritic spine; pink, axon terminal; dark blue, altered mitochondria; purple, immature lysosomes; and yellow, mature lysosomes. Data are expressed as mean ± S.E.M. For normally distributed data, two-tailed paired Student’s t-test was used (C, D, F–I), while for non-normally distributed data the paired Wilcoxon test was used (E). Levels of significance were set to: *p < 0.05, **p < 0.01

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