Fig. 3

Amoeboid microglia proliferate and transition to a ramified state in the CX3CR1^Cre−ER:R26^iDTR retina. A, B Quantification of Iba1⁺ cells/mm³ (A) and confocal images of retinas for Iba1 (green) (B). C, D Quantification of EdU⁺ cells/mm³ within the Iba1⁺ cell population (C) and confocal images of retinas for Iba1 (green), EdU (red) and DAPI (blue) in diabetic-PBS and diabetic-DTx mice (D). E Transformation index in non-diabetic, and diabetic mice PBS and DTx-treated mice, n = 98 to 150 microglia per group where each dot represents an individual microglia cell and bars show mean ± SD. F Representation of cellular tracings from transformation index quantification. Data show mean ± SD, n = 5–9 mice per group where each dot represents an individual mouse (A, C). *P < 0.05, ***P < 0.001, ****P < 0.0001 using Student’s t-test, with Welch’s correction