Fig. 4
Prolonged DTx exposure and recovery prevents neurodegeneration and vascular damage in the diabetic CX3CR1Cre−ER:R26iDTR retina. A Confocal images of retinal tissues stained for Iba1 (green), NeuN and RBPMS (red-top panel), TUJ1 (turquoise), GFAP (purple), CD31 (red-bottom panel) and fibrinogen (white) in non-diabetic diabetic-PBS, diabetic-DTx, diabetic PBS-recovery and diabetic DTx-recovery mice. B–F Quantification of retinal IHC analysis for GFAP+ immunoreactivity (B), NeuN+RBPMS+ cells/mm3 (C), and percent immunoreactive area for TUJ1 (D), CD31 (E), and fibrinogen (F). Data show mean ± SD, n = 4–9 mice per group where each dot represents an individual mouse. *P < 0.05, **P < 0.01, ***P < 0.001 ****P < 0.0001 using Student’s t-test, with Welch’s correction