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Fig. 6 | Journal of Neuroinflammation

Fig. 6

From: Parabiosis reveals the correlation between the recruitment of circulating antigen presenting cells to the retina and the induction of spontaneous autoimmune uveoretinitis

Fig. 6

Flow cytometry analysis of retinal CD11b cells from parabiotic recipient mice. Donor mice are R161H+/− × CD11cDTR/GFP F1 joined to B10.RIII × B6/J F1 (wild type) recipients. A CD11b+ cells from representative parabiotic wild-type recipient (top), unpaired prophase R161H+/− × CD11cDTR/GFP F1 (middle), and control CD11cDTR/GFP (bottom) retinas. Retinal CD45+ cells were selected as described in Fig. 4 with CD45+Ly6Gneg cells further gated to select for CD11b cells (CD11b+CD49bnegCD3negCD4neg). CD11b+ cells were then analyzed for CD45 expression levels versus SSC-H with CD45med and CD45hi cell populations and overall average percentage indicated. B Representative analysis of retinal CD11b+CD45med and CD11b+CD45hi cells for GFP expression with cell numbers indicated. C Graphical comparison of GFP expression in CD11b+CD45hi cells from unpaired (control) CD11cDTR/GFP mice (n = 6), unpaired (control) R161+/− × CD11cDTR/GFP F1 mice (n = 4), and parabiotic wild-type recipient mice (n = 4). Data shown as mean ± SD, * = ρ < 0.05 by t test

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