Fig. 6From: Parabiosis reveals the correlation between the recruitment of circulating antigen presenting cells to the retina and the induction of spontaneous autoimmune uveoretinitisFlow cytometry analysis of retinal CD11b cells from parabiotic recipient mice. Donor mice are R161H+/− × CD11cDTR/GFP F1 joined to B10.RIII × B6/J F1 (wild type) recipients. A CD11b+ cells from representative parabiotic wild-type recipient (top), unpaired prophase R161H+/− × CD11cDTR/GFP F1 (middle), and control CD11cDTR/GFP (bottom) retinas. Retinal CD45+ cells were selected as described in Fig. 4 with CD45+Ly6Gneg cells further gated to select for CD11b cells (CD11b+CD49bnegCD3negCD4neg). CD11b+ cells were then analyzed for CD45 expression levels versus SSC-H with CD45med and CD45hi cell populations and overall average percentage indicated. B Representative analysis of retinal CD11b+CD45med and CD11b+CD45hi cells for GFP expression with cell numbers indicated. C Graphical comparison of GFP expression in CD11b+CD45hi cells from unpaired (control) CD11cDTR/GFP mice (n = 6), unpaired (control) R161+/− × CD11cDTR/GFP F1 mice (n = 4), and parabiotic wild-type recipient mice (n = 4). Data shown as mean ± SD, * = ρ < 0.05 by t testBack to article page