Fig. 5

SIRT5 suppresses ANXA1 secretion after OGD/R. A, B Primary cultured microglia were infected with adenovirus encoding HA-ANXA1-WT, HA-ANXA1-166R or HA-ANXA1-166E and subjected to OGD/R. ELISA analysis indicating the secretion level of ANXA1 (A). Co-IP assays showed the binding of ectopically expressed ANXA1 with endogenous FPR2 (B). C, D Primary cultured microglia were infected with adenoviruses encoding Flag-SIRT5-WT and Flag-SIRT5-H158Y and subjected to OGD/R. ELISA analysis indicating the secretion level of ANXA1 (C). Co-IP assays showed the binding of endogenous ANXA1 with FPR2 (D). E, F Primary cultured microglia were infected with adenovirus encoding SIRT5 shRNA sequence #1 or #2 and subjected to OGD/R. ELISA analysis indicating the secretion level of ANXA1 (E). Co-IP assays showing the binding of endogenous ANXA1 with FPR2 (F). Data are presented as the mean ± SEM from at least three independent experiments. ns: no significance, *P < 0.05, **P < 0.01, ***P < 0.001