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Fig. 6 | Journal of Neuroinflammation

Fig. 6

From: Sirtuin 5 aggravates microglia-induced neuroinflammation following ischaemic stroke by modulating the desuccinylation of Annexin-A1

Fig. 6

SIRT5-induced desuccinylation of ANXA1 decreased its SUMOylation level. A, B Primary cultured microglia were infected with adenovirus encoding HA-ANXA1-WT, HA-ANXA1-166R or HA-ANXA1-166E. A co-IP assay was performed to confirm the SUMOylation levels of ANXA1 (A) and the interaction of ectopically expressed ANXA1 with SENP6 (B). C, D Primary cultured microglia were infected with adenovirus encoding SIRT5-WT or SIRT5-HY. Co-IP confirmed the SUMOylation levels of ANXA1 (C) and the interaction of endogenous ANXA1 with SENP6 (D). E, F Primary cultured microglia were infected with adenovirus encoding shRNAs against SIRT5 or nontargeted scramble control. A co-IP assay was performed to confirm the SUMOylation levels of ANXA1 (E) and the interaction of endogenous ANXA1 with SENP6 (F). Data represent at least three independent experiments

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Journal of Neuroinflammation

ISSN: 1742-2094