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Fig. 8 | Journal of Neuroinflammation

Fig. 8

From: Sirtuin 5 aggravates microglia-induced neuroinflammation following ischaemic stroke by modulating the desuccinylation of Annexin-A1

Fig. 8

SIRT5 knockdown in microglia protects against OGD/R-induced neuronal damage. A Primary microglial cells were transduced with recombinant adenovirus carrying the SIRT5 coding sequence or shRNA sequence for 48 h and co-cultured with neurons via a Transwell system, followed by OGD/R treatment. Schematic representation as above. B, C Neuronal apoptosis was determined by TUNEL staining (B), and quantitative analysis was conducted to show the percentage of TUNEL+ neurons (C). D LDH release showing neuronal damage. E CCK-8 assay indicating neuronal viability. F–I Western blot assays (F) and quantification analysis showing the protein levels of cleaved caspase-3 (G), cleaved caspase-9 (H), and cleaved PARP (I) in neurons. Data are presented as the mean ± SEM from five independent experiments. **P < 0.01, ***P < 0.001 and ****P < 0.0001

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