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Fig. 9 | Journal of Neuroinflammation

Fig. 9

From: Sirtuin 5 aggravates microglia-induced neuroinflammation following ischaemic stroke by modulating the desuccinylation of Annexin-A1

Fig. 9

SIRT5 knockdown in microglia is neuroprotective during cerebral I/R injury. A Experimental design for microglia-specific overexpression and knockdown of SIRT5. AAV2/6 vectors encoding CMV-DIO-His-SIRT5 or CMV-DIO-EGFP-U6-shSIRT5 were stereotactically injected into the hippocampal CA1 region, cerebral cortex and striatum of Cx3cr1-Cre mice. B A diagram of the experimental process. C, D TTC staining indicating the infarction volume induced by ischaemic stroke (C) and quantification analysis showing the infarct size (D). n = 9 mice per group. E The modified neurological severity score (mNSS) showing the neurological deficit scores after ischaemic stroke. n = 9 mice per group. F–J Latency trial (F, G) and probe trial (H–J) tests in the Morris water maze (MWM). n = 8–10 mice per group. F The mean escape latency of the MWM tests during Days 1–6 of testing. G The mean escape latency to the hidden platform on Day 6 in the MWM tests. H Time (in seconds, s) spent in the target quadrant on Day 7. I T Number of times crossing the target platform location on Day 7. J Representative path tracing on Day 7 of the probe test. K, L Novel object recognition (NOR) testing. K The time spent exploring two identical objects of the mice in the familiarization trial. L The percentage of time spent exploring the novel and familiar objects in the test trial. n = 8–10 mice per group. Data are presented as the mean ± SEM. ns: no significance, *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001

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